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Evonik
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Dyesol Inc
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Journal: Chembiochem
Article Title: Biomimetic Compartmentalization of Enzymes for Sustainable Coenzyme Recycling in Oxidative Photocatalysis
doi: 10.1002/cbic.70381
Figure Lengend Snippet: The compartmentalized enzyme‐photocatalyst system for sustainable coenzyme regeneration. (a) The glucose dehydrogenase‐loaded silica nanoparticles (GDH@SiNPs) and TiO 2 nanoparticle system complete the NAD + /NADH cycle, enabling continuous coenzyme regeneration while preventing enzyme inactivation by reactive oxygen species generated during photocatalysis. (b) Bare GDH is easily deactivated by the reaction of TiO 2 nanoparticles. (c) The silica matrix quenched ROS before reaching the enzyme core due to their short half‐life in an aqueous environment, effectively shielding GDH from oxidative inactivation.
Article Snippet:
Techniques: Generated
Journal: Chembiochem
Article Title: Biomimetic Compartmentalization of Enzymes for Sustainable Coenzyme Recycling in Oxidative Photocatalysis
doi: 10.1002/cbic.70381
Figure Lengend Snippet: Oxidative stress resistance, and recycling of NAD + and NADH by alternating addition of glucose in the dark and irradiation with UV light. (a) NAD + and NADH conversion in this study. (b) Monitoring of GDH activity in the presence of ROS (generated by TiO 2 nanoparticles under UV light irradiation) ( n = 3). (c) Recycling of NAD + and NADH using GDH@SiNPs and TiO 2 nanoparticles ( n = 3). (d) Recycling of NAD + and NADH using native GDH and TiO 2 nanoparticles. For c and d, an increase in the y ‐axis value indicates NADH accumulation by GDH‐catalyzed reduction, while a decrease reflects NAD + regeneration by TiO 2 ‐mediated oxidation ( n = 3).
Article Snippet:
Techniques: Irradiation, Activity Assay, Generated
Journal: Chembiochem
Article Title: Biomimetic Compartmentalization of Enzymes for Sustainable Coenzyme Recycling in Oxidative Photocatalysis
doi: 10.1002/cbic.70381
Figure Lengend Snippet: Cell protection of GDH@SiNPs against TiO2‐triggered ROS stress. (a) GDH@SiNPs can mitigate oxidative stress by continuously supplying NADH, which serves as a direct ROS scavenger. (b) Cell viability measured by CCK‐8 assay of HeLa cells in the presence of GDH@SiNPs and TiO2 nanoparticles under UV light irradiation. Effective cell protection of the GDH@SiNPs was achieved by enzymatic NADH production ( n = 4). Data are presented as mean ± SD ( n = 4). Statistical significance was determined by one‐way ANOVA followed by Tukey's post‐hoc test. Significant differences are indicated as **( p < 0.01), and ***( p < 0.001).
Article Snippet:
Techniques: CCK-8 Assay, Irradiation